Spermiogenesis: Phases And Their Characteristics

The spermatogenesis , spermatic also known as metamorphosis, corresponds to the transformation process spermatids (or spermatids) in mature spermatozoa. This phase occurs when spermatids are attached to Sertoli cells.

In contrast, the term spermatogenesis refers to the production of haploid spermatozoa (23 chromosomes) from undifferentiated and diploid spermatogonia (46 chromosomes).

The spermatids of a mammal are characterized by having a rounded shape and lacking a flagellum, which is the whip-shaped appendix that helps movement, typical of sperm. The spermatids must mature into a sperm capable of performing its function: reaching the ovum and joining it.

Therefore, they must develop a flagellum morphologically reorganizing, thus acquiring motility and interaction capacity. The phases of spermiogenesis were described in 1963 and 1964 by Clermont and Heller, thanks to the visualization of each of the changes using light microcopy in human tissues.

The sperm differentiation process that occurs in mammals involves the following stages: the construction of an acrosomal vesicle, the formation of a hood, rotation and condensation of the nucleus.

Phases

Golgi phase

Periodic acid granules, Schiff’s reagent, abbreviated PAS, accumulate in the Golgi complex of spermatids.

Acrosomal vesicle

PAS granules are rich in glycoproteins (proteins bound to carbohydrates) and will give rise to a vesicular structure called the acrosomal vesicle. During the Golgi phase, this vesicle increases in size.

The polarity of the sperm is defined by the position of the acrosomal vesicle and this structure will be located in the anterior pole of the sperm.

The acrosome is a structure that contains hydrolytic enzymes, such as hyaluronidase, trypsin and acrosin, whose function is the disintegration of the cells that accompany the oocyte, hydrolyzing the components of the matrix, such as hyaluronic acid.

This process is known as an acrosomal reaction and it begins with the contact between the sperm and the outermost layer of the oocyte, called the zona pellucida.

Centriole migration

Another key event of the Golgi phase is the migration of the centrioles to the posterior region of the spermatid, and their alignment with the plasma membrane occurs .

The centriole proceeds to the assembly of the nine peripheral microtubules and the two central ones that make up the sperm flagellum.

This set of microtubules are capable of transforming energy – ATP (adenosine triphosphate) generated in the mitochondria – into movement.

Cap phase

The acrosomal vesicle proceeds to expand towards the anterior half of the cell nucleus , giving the appearance of a helmet or cap. In this area, the nuclear envelope degenerates its pores and the structure thickens. Also, core condensation occurs.

Major changes in the core

During spermiogenesis, a series of transformations of the nucleus of the future sperm occurs, such as compaction to 10% of the initial size and the replacement of histones by protamines.

Protamines are proteins of about 5000 Da, rich in arginine, with less lysine, and soluble in water. These proteins are common in the sperm of different species and help in the extreme condemnation of the DNA in an almost crystalline structure.

Acrosome phase

A change of orientation of the spermatid occurs: the head is disposed towards the Sertoli cells and the flagellum — in the process of development — extends into the seminiferous tube.

The already condensed nucleus changes its shape, lengthening and taking on a more flattened shape. The nucleus, together with the acrosome, travels close to the plasma membrane at the anterior end.

In addition, a reorganization of the microtubules occurs into a cylindrical structure that widens from the acrosome to the posterior end of the spermatid.

As for the centrioles, after completing their function in the development of the flagellum, they return to the posterior area of ​​the nucleus and adhere to it.

Formation of the connecting piece

A series of modifications occurs to form the “neck” of the sperm. From the centrioles, now attached to the nucleus, emerge nine fibers of a significant diameter that spread in the tail outside the microtubules.

Note that these dense fibers join the nucleus with the flagellum; This is why it is known as a “connecting piece”.

Formation of the intermediate piece

The plasma membrane shifts to envelop the developing flagellum, and the mitochondria shift to form a helical structure around the neck that extends to the immediate posterior region.

The newly formed region is called the middle piece, located in the tail of the sperm. Likewise, the fibrous sheath, the main part and the main part can be distinguished.

The mitochondria originate a continuous covering that surrounds the intermediate piece, this layer has the shape of a pyramid and participates in the generation of energy and in sperm movements.

Ripening phase

The excess of cellular cytoplasmic content is phagocytosed by Sertoli cells, in the form of residual bodies.

Final morphology

After spermiogenesis, the sperm has radically changed its shape and is now a specialized cell capable of movement.

In the spermatozoa generated, the head region can be differentiated (2–3 um in width and 4 to 5 um in length), where the cell nucleus with the haploid genetic load and the acrosome are located.

After the head is the intermediate region, where the centrioles, the mitochondrial helix and the tail of about 50 um in length are located.

The spermiogenesis process varies depending on the species, although on average it lasts from one to three weeks. In experiments in mice, the sperm formation process takes 34.5 days. In contrast, the process in humans takes almost twice as long.

Spermatogenesis is a complete process that can occur continuously, generating about 100 million sperm per human testicle each day.

The release of sperm by ejaculation involves about 200 million. During his entire life, a man can produce 10 12 to 10 13 sperm.

References

  1. Carlson, BM (2005). Human embryology and developmental biology . Elsevier.
  2. Cheng, CY, & Mruk, DD (2010). The biology of spermatogenesis: the past, present and future. Philosophical Transactions of the Royal Society B: Biological Sciences , 365 (1546), 1459–1463.
  3. Gilbert SF. (2000) Developmental Biology. 6th edition . Sunderland (MA): Sinauer Associates. Spermatogenesis. Available from: ncbi.nlm.nih.gov/books/NBK10095
  4. González – Merlo, J., & Bosquet, JG (2000). Oncological gynecology . Elsevier Spain.
  5. Larsen, WJ, Potter, SS, Scott, WJ, & Sherman, LS (2003). Human embryology . Elsevier ,.
  6. Ross, MH, & Pawlina, W. (2007). Histology. Text and Color Atlas with Cellular and Molecular Biology (Includes Cd – Rom) 5aed . Panamerican Medical Ed.
  7. Urbina, MT, & Biber, JL (2009). Fertility and assisted reproduction . Panamerican Medical Ed.
  8. Wein, AJ, Kavoussi, LR, Partin, AW, & Novick, AC (2008). Campbell – Walsh Urology. Panamerican Medical Ed.

Add a Comment

Your email address will not be published. Required fields are marked *